Effect of cell cycle arrest on intermediate metabolism in the marine diatom Phaeodactylum tricornutum.
Identifieur interne : 000D58 ( Main/Exploration ); précédent : 000D57; suivant : 000D59Effect of cell cycle arrest on intermediate metabolism in the marine diatom Phaeodactylum tricornutum.
Auteurs : Joomi Kim [États-Unis] ; Christopher M. Brown [États-Unis] ; Min Kyung Kim [États-Unis] ; Elizabeth H. Burrows [États-Unis] ; Stéphane Bach [France] ; Desmond S. Lun [États-Unis] ; Paul G. Falkowski [États-Unis]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2017.
Abstract
The inhibitor NU 2058 [6-(cyclohexylmethoxy)-9H-purin-2-amine] leads to G1-phase cell cycle arrest in the marine diatom, Phaeodactylum tricornutum, by binding to two cyclin-dependent kinases, CDKA1 and CDKA2. NU 2058 has no effect on photosynthetic attributes, such as Fv/Fm, chlorophyll a/cell, levels of D2 PSII subunits, or RbcL; however, cell cycle arrest leads to unbalanced growth whereby photosynthetic products that can no longer be used for cell division are redirected toward carbohydrates and triacylglycerols (TAGs). Arrested cells up-regulate most genes involved in fatty acid synthesis, including acetyl-CoA carboxylase, and three out of five putative type II diglyceride acyltransferases (DGATs), the enzymes that catalyze TAG production. Correlation of transcriptomes in arrested cells with a flux balance model for P. tricornutum predicts that reactions in the mitochondrion that supply glycerate may support TAG synthesis. Our results reveal that sources of intermediate metabolites and macromolecular sinks are tightly coupled to the cell cycle in a marine diatom, and that arresting cells in the G1 phase leads to remodeling of intermediate metabolism and unbalanced growth.
DOI: 10.1073/pnas.1711642114
PubMed: 28874574
Affiliations:
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Falkowski</name><affiliation wicri:level="1"><nlm:affiliation>Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901; falko@marine.rutgers.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick</wicri:regionArea><wicri:noRegion>New Brunswick</wicri:noRegion></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The inhibitor NU 2058 [6-(cyclohexylmethoxy)-9H-purin-2-amine] leads to G1-phase cell cycle arrest in the marine diatom, Phaeodactylum tricornutum, by binding to two cyclin-dependent kinases, CDKA1 and CDKA2. NU 2058 has no effect on photosynthetic attributes, such as Fv/Fm, chlorophyll a/cell, levels of D2 PSII subunits, or RbcL; however, cell cycle arrest leads to unbalanced growth whereby photosynthetic products that can no longer be used for cell division are redirected toward carbohydrates and triacylglycerols (TAGs). Arrested cells up-regulate most genes involved in fatty acid synthesis, including acetyl-CoA carboxylase, and three out of five putative type II diglyceride acyltransferases (DGATs), the enzymes that catalyze TAG production. Correlation of transcriptomes in arrested cells with a flux balance model for P. tricornutum predicts that reactions in the mitochondrion that supply glycerate may support TAG synthesis. Our results reveal that sources of intermediate metabolites and macromolecular sinks are tightly coupled to the cell cycle in a marine diatom, and that arresting cells in the G1 phase leads to remodeling of intermediate metabolism and unbalanced growth.</div></front></TEI><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>New Jersey</li><li>Région Bretagne</li></region><settlement><li>Roscoff</li></settlement></list><tree><country name="États-Unis"><region name="New Jersey"><name sortKey="Kim, Joomi" sort="Kim, Joomi" uniqKey="Kim J" first="Joomi" last="Kim">Joomi Kim</name></region><name sortKey="Brown, Christopher M" sort="Brown, Christopher M" uniqKey="Brown C" first="Christopher M" last="Brown">Christopher M. Brown</name><name sortKey="Burrows, Elizabeth H" sort="Burrows, Elizabeth H" uniqKey="Burrows E" first="Elizabeth H" last="Burrows">Elizabeth H. Burrows</name><name sortKey="Falkowski, Paul G" sort="Falkowski, Paul G" uniqKey="Falkowski P" first="Paul G" last="Falkowski">Paul G. Falkowski</name><name sortKey="Kim, Min Kyung" sort="Kim, Min Kyung" uniqKey="Kim M" first="Min Kyung" last="Kim">Min Kyung Kim</name><name sortKey="Lun, Desmond S" sort="Lun, Desmond S" uniqKey="Lun D" first="Desmond S" last="Lun">Desmond S. Lun</name></country><country name="France"><region name="Région Bretagne"><name sortKey="Bach, Stephane" sort="Bach, Stephane" uniqKey="Bach S" first="Stéphane" last="Bach">Stéphane Bach</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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